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CS-W001157 4
Total: USD 88.00

ONPG

  • CAS No. 369-07-3

  • Cat. No. CS-3045
  • Molecule weight301.25
  • Purity≥97%
  • MDL No.MFCD00063255

*For research and further manufacturing use only, not for direct human use.

ONPG|CS-3045

Structure of 369-07-3

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General Information

  • CAS No. 369-07-3
  • Cat. No. CS-3045
  • Purity ≥97%
  • MDL No. MFCD00063255
  • Storage 4°C
  • Shipping Room temperature in continental US; may vary elsewhere.
  • Molecular Formula C₁₂H₁₅NO₈
  • Molecular Weight 301.25
  • Synonym(s) 2-Nitrophenyl β-D-galactopyranoside
  • SMILES O[C@H]([C@@H](O)[C@H]1O)[C@@H](O[C@@H]1CO)OC2=C([N+]([O-])=O)C=CC=C2

Description

Synthesizing glycosidase substrates, 2-Nitrophenyl β-D-galactopyranoside (CAS 369-07-3) enables quantitative monitoring of enzymatic hydrolysis through chromogenic release of 2-nitrophenol in kinetic assays.

Application

2-Nitrophenyl β-D-galactopyranoside (CAS 369-07-3) drives β-galactosidase activity assays by releasing yellow o-nitrophenol upon enzymatic cleavage, enabling real-time spectrophotometric monitoring at 420 nm. This setup streamlines gene expression studies using lacZ reporter systems. Researchers leverage its reliable chromogenic response for enzyme kinetics, microbial screening, and high-throughput functional analysis across molecular biology and biochemical research.

Safety Information

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GHS Pictogram :
GHS No : GHS07
Signal Word : Warning
UN#: N/A
Class : N/A
Packing Group : N/A
Hazard Statements : H302-H315-H319-H335
Precautionary Statements : P261-P264-P270-P271-P280-P302+P352-P304+P340-P305+P351+P338-P330-P362+P364-P403+P233-P405-P501

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Computational Chemistry Data

  • TPSA   142.52
  • LogP   -1.2265
  • H_Acceptors   8
  • H_Donors   4
  • Rotatable_Bonds   4

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