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Structure of 7414-83-7
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Dithiothreitol (DTT) is a disulfide (SH) reducing agent found in proteins and in the amino acid cysteine. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange. It is almost 7-fold stronger reducing agent than ME, hence efficient in lower concentrations.DTT has been used:as one of the reactants in the reduction and alkylation of s1-Casein, the major allergen of cows milk.as a component of medium for the demembranation and reactivation of spermatozoa.to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.in proteomics analysis as in-solution protein digestion for mass spectrometry as a buffer component for protein quantification, to prepare wash buffer, lysis buffer, sample buffer, and protein elution buffer
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GHS Pictogram :
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GHS No : GHS07
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Signal Word : Warning
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UN#: N/A
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Class : N/A
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Packing Group : N/A
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Hazard Statements : H302
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Precautionary Statements : P264-P270-P330-P501
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Lot numbers can be found on the outside label of a product: