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Structure of 720707-93-7
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Tween20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.TWEEN 20 is widely used in biochemical applications. It has been used:As an emulsifying agent for the preparation of stable oil-in-water emulsionsIn pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%For lysing mammalian cells at a concentration of 0.005 to 0.5%Along with PBS in the dilution of antibodies in immunohistochemistry techniqueFor washing cells in FISH (Fluorescence in situ hybridization) technique
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GHS Pictogram :
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GHS No : GHS07
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Signal Word : Warning
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UN#: N/A
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Class : N/A
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Packing Group : N/A
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Hazard Statements : H302-H315-H319-H335
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Precautionary Statements : P261-P264-P270-P271-P280-P302+P352-P304+P340-P330-P362+P364-P405-P501
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Lot numbers can be found on the outside label of a product: