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Structure of 86770-74-3
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MES hydrate buffer (2-(N-morpholino)ethanesulfonic acid monohydrate) is a zwitterionic biological buffer widely employed in biological, environmental, and biochemical research. With a pKa of 6.1, it is the go-to choice for buffering solutions at physiological pH. MES was developed as one of Goods buffers in the 1960s, meeting specific criteria: a midrange pKa, maximum water solubility, minimal solubility in other solvents, minimal salt effects, stability in different temperatures, chemical and enzymatic stability, minimal absorption in the visible and UV spectral range, and ease of synthesis. MES Monohydrates attributes, such as low UV absorptivity, minimal reactivity, pH stability, and water solubility, position it as a Goods buffer.MES hydrate buffer also exhibits high water solubility and minimal metal ion binding, making it an ideal choice for various applications:Cell Biology: MES hydrate buffer is often the preferred choice for buffering cell culture media due to its lower toxicity to cells compared to other buffers like Tris and phosphate.Protein Purification and Extraction: Its stability across a wide pH range and minimal metal ion binding make MES hydrate buffer a valuable asset in protein purification and extraction protocols.Gel Electrophoresis: MES hydrate buffer is also commonly used as a running buffer in denaturing gel electrophoresis, ensuring reliable results in protein separation and analysis.MES Hydrate has been used:As a component of extraction buffer in the extraction of the enzyme BACE1As a component of the reaction mixture for the measurement of 5,10-methenyltetrahydrofolate synthetase (MTHFS) enzyme activity in fibroblastsAs a component of MES coupling buffer during the coupling of mAb or GST-fusion proteins to carboxylate-modified polystyrene latex (CML) beadsto adjust the pH of the solution for the fluorescent labelingas a component of electrophoresis buffer
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GHS Pictogram :
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GHS No : GHS07,GHS05
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Signal Word : Danger
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UN#: N/A
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Class : N/A
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Packing Group : N/A
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Hazard Statements : H315-H318-H335
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Precautionary Statements : P260-P264-P271-P280-P302+P352-P304+P340-P305+P351+P338-P310-P312-P332+P313-P362+P364-P403+P233-P405-P501
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Lot numbers can be found on the outside label of a product: